溶液配制: Honda buffer: 25 mM Tris-HCl(pH7.4) 10 mM MgCl2 2.5% (wt/vol) Ficoll 400 5% (wt/vol) Dextran T 40 0.4 M蔗糖 1x Protease Inhibitor Cocktail (用前加) 5 mM DTT (用前加) 10 mM β-巯基乙醇 (用前加) Triton X-100:20% (vol/vol) 试剂及耗材: Triton X-100:#T8787, Sigma, St. Louis, USA 蛋白酶抑制剂(cOmplete Protease Inhibitor Cocktail Tablets): #11836145001, Roche, Mannheim, Germany 二硫苏糖醇(DL-dithiothreitol, DTT): AMRESCO, Solon, USA Ficoll 400